Previously this season, Dubois hit .217 with four home runs, six doubles and 18 RBIs in 33 games with Class AAA Columbus of the International League.
The Cubs said Tuesday they were happy to employ Dubois again.
"He just recently got released and I always told him that if he ever needed anything he would be welcomed back in the club," Cubs Vice President of Player Personnel Oneri Fleita said.
With Daryle Ward going on the 15-day disabled list and Micah Hoffpauir being brought up from Iowa earlier this week, a spot was open for Dubois.
In Hoffpauir's absence, the Cubs could have promoted Jake Fox or Matt Craig from Class AA Tennessee, but chose not to "because I want Jake to play every day, coupled with the fact that Micah could return once Ward is healthy," Fleita said.
"It is not the way to treat the prospect," he added.
InsideTheIvy.com staff writer Corey Ann Dobridnia in Daytona Beach contributed to this report.